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Clinical Chemistry 19: 1016-1021, 1973;
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Clinical Chemistry, Vol 19, 1016-1021, Copyright © 1973 by the American Association for Clinical Chemistry

Rapid Radioimmunoassay of Triiodothyronine

Christine B. Sekadde 1, W. Roy Slaunwhite Jr. 1, and Thomas Aceto Jr. 1

1 Departments of Biochemistry and Pediatrics, State University of New York at Buffalo, Endocrine Laboratories of Children’s Hospital of Buffalo, Buffalo, N. Y. 14222.

The method used in this report is based on the immunological production of a specific antisera to triiodothyronine (T3) in rabbits. Conducted at 4 °C, the radioimmunoassay requires three to five days to complete. Hoping to speed this assay, we investigated the influence of time, temperature, and pH. Incubation at 37 °C and pH 8.0 for 30 min did not deleteriously affect either precision, specificity, reproducibility, or accuracy. Bound and unbound T3 were separated in 10 min by adding polyethylene glycol ("Carbowax 6000") at 0-4 °C. T3 was prevented from binding to thyroxine-binding globulin by the addition of 8-anilino-1-naphthalene sulfonic acid. To minimize the differences in protein concentration between standards and unknowns, we added T3-free serum to all standards. Thus, a simplified and convenient method is described for measurement of T3 that is suitable for clinical use because it is simpler and faster than previously reported methods.


Key Words: assessment of thyroid function • thyrotoxicosis • hyper- and hypothyroidism

Submitted on May 3, 1973
Accepted on June 18, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.