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Clinical Chemistry 19: 1053-1057, 1973;
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Clinical Chemistry, Vol 19, 1053-1057, Copyright © 1973 by the American Association for Clinical Chemistry

Standard Reference Materials: Standard Quartz Cuvettes for High-Accuracy Spectrophotometry

R. Mavrodineanu 1 and J. W. Lazar 1

1 Analytical Chemistry Division, Institute for Materials Research, National Bureau of Standards, Washington, D. C. 20234; and the Optical Physics Division, Institute for Basic Standards, National Bureau of Standards, Washington, D. C. 20234.

Accurate knowledge of lightpath and parallelism of cuvettes used in spectrophotometry are indispensable parameters that must be determined when accurate transmittance measurements of liquid materials are considered. A description is given of the design and techniques developed at NBS for production of quartz cuvettes (SRM 932) having a nominal radiation pathlength of 10 mm ± 0.03 mm. For each cuvette, pathlength and parallelism are certified with an uncertainty of ±0.0005 mm. The method and instrumentation used to determine these parameters are also described.


Key Words: lightpath • instrumental pathlength • radiation pathlength • variables in analysis

Submitted on June 22, 1973
Accepted on July 6, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.