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Clinical Chemistry 19: 1063-1066, 1973;
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Clinical Chemistry, Vol 19, 1063-1066, Copyright © 1973 by the American Association for Clinical Chemistry

Radioimmunoassay of Thyroxine in Unextracted Serum, by a Single-Antibody Technique

Ralph T. Dunn 1 and Lowell B. Foster 1

1 Patterson Coleman Laboratories, 4600 N. Habana, Tampa, Fla. 33614.

A radioimmunoassay procedure for thyroxine (T4) is described, for which only 25 µl of serum is required. The antibody, commercially available, is produced in rabbits in response to injections of a T4—albumin conjugate. Native T4—binding proteins are denatured by heating 25 µl of serum in 0.2 ml of a glutamate buffer in a boiling water bath. After a 1-h incubation of heat-treated serum with I125-T4 and T4 antibody, "free" and "bound" T4 are separated by use of hemoglobin-coated charcoal. The method has excellent sensitivity, is rapid, and possesses significant advantages over a standard competitive protein binding method with which it is compared.


Key Words: results for T4 by competitive protein binding compared • assessment of thyroid function • T4—albumin conjugate as antigen • normal range

Submitted on May 18, 1973
Accepted on July 5, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.