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Clinical Chemistry 19: 1077-1078, 1973;
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Clinical Chemistry, Vol 19, 1077-1078, Copyright © 1973 by the American Association for Clinical Chemistry

Improved Manual Spectrophotometric Procedure for Determination of Serum Triglycerides

Sidney P. Gottfried 1 and Bernard Rosenberg 1

1 Stamford Medical Laboratory, 810 Bedford St., Stamford, Conn. 06901.

We have modified the procedure of Levy [Ann. Clin. Lab. Sci. 6, 474 (1972)] for determination of serum triglycerides. The changes consist of using heptane in place of nonane; using concentrated aqueous potassium hydroxide instead of sodium methylate as the saponifying reagent; and a somewhat higher saponification, oxidation, and condensation temperature. Consequently, the yellow dehydrolutidine derivative is determined in a homogeneous solution having an almost linear absorbance up to 3 g/liter.


Key Words: colorimetry of dehydrolutidine derivative of triglycerides

Submitted on July 31, 1972
Accepted on June 19, 1973




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