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Clinical Chemistry 19: 973-976, 1973;
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Clinical Chemistry, Vol 19, 973-976, Copyright © 1973 by the American Association for Clinical Chemistry

Evaluation and Recalculation of Manufacturer’s Instrument-Specific Aspartate Aminotransferase Values for Reference Sera

Ronald H. Laessig 1, Andris Indriksons 1, Thomas H. Schwartz 1, and Toni A. Paskey 1

1 State Laboratory of Hygiene University of Wisconsin Medical Center, Madison, Wis. 53706; and the Multiphasic Health Screening Program, Department of Health and Social Services, Madison, Wis. 53706.

We compared a manufacturer’s stated values to those obtained by a reference method for aspartate aminotransferase activity in reference sera. We determined the validity with which this manufacturer’s stated values may be used as multichannel-analyzer set points, and related values obtained by the reference method to instrument-specific values by regression analysis. Data were obtained from 40 multichannel analyzers, comprising three different instruments (and their respective procedures): SMA 12/ 60, ultraviolet and colorimetric; SMA 12/30, colorimetric (reported in Karmen units); and MARK X, colorimetric. Results that best correspond with those for the Karmen-uv reference method are those obtained by automated methods that most closely emulate it. Regression equations relate Karmen (reference method) results to instrument-specific values. These relationships enable laboratories to check assay values of their lots of reference or control sera against a consensus value obtained from the group of similar instruments used in the study. Regression equations are calculated for the intercomparison of results from the four automated procedures.


Key Words: results from automated instrumentation intercompared • Hycel MARK X • SMA 12/30 and 12/60 • colorimetric and uv methods for aspartate aminotransferase • method of Karmen

Submitted on December 28, 1972
Accepted on June 4, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.