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Clinical Chemistry 2: 125-130, 1956;
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Clinical Chemistry, Vol 2, 125-130, Copyright © 1956 by the American Association for Clinical Chemistry

Minor Hemoglobins in Erythrocytes

Demonstration and Analysis by Paper Electrophoresis

H. Hoch 1 and G. H. Barr 1

1 department of Biophysics, Medical College of Virginia, Richmond.

Two minor hemoglobins present in normal human erythrocytes have been separated by paper electrophoresis.

The concentration of the component which was more positively charged than the bulk hemoglobin ranged between 1.5 and 6 per cent of the total hemoglobin. The concentration of the other, more negatively charged, component could not be measured on account of incomplete separation, but was estimated to range from very low values to 5 per cent.

Globin added to the buffer was found to suppress the irreversible adsorption of hemoglobin on the filter paper and thus to eliminate "tailing."

Submitted on November 15, 1955






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Copyright © 1956 by the American Association for Clinical Chemistry.