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Clinical Chemistry 2: 65-74, 1956;
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Clinical Chemistry, Vol 2, 65-74, Copyright © 1956 by the American Association for Clinical Chemistry

Cystine in Human Serum Proteins

Miriam Reiner 1 and Michael X. Sullivan 1

1 Chemistry Laboratory, District of Columbia General Hospital, and the ChemoMedical Research Institute, Georgetown University, Washington, D. C.

1. The amount of cystine was determined in a number of serum protein fractions separated by the procedure of E. J. Cohn. In albumin, the average value was 6.40 per cent for cystine; for gamma-globulin the average value was 1.94 per cent; the other fractions tested were mixtures and varied according to the different globulins present. Globin from human hemoglobin contained 1.90 per cent cystine.

2. Separated fractions from three types of multiple myeloma cases have been presented showing abnormal fractions in the alpha-, beta-, and gammaregions. The percentage of cystine was determined, and except for the fraction from the "gamma" type of myelomas, which contained 6.15 per cent cystine, the anomalous protein fractions contained about the same amount found in separated fractions of normal serum.






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Copyright © 1956 by the American Association for Clinical Chemistry.