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Clinical Chemistry 2: 99-111, 1956;
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Clinical Chemistry, Vol 2, 99-111, Copyright © 1956 by the American Association for Clinical Chemistry

Buffer Composition in Paper Electrophoresis

Considerations on its Influence, with Special Reference to the Interaction Between Small Ions and Proteins

C. B. Laurell 1, S. Laurell 1, and Nancy Skoog 1

1 Laboratories of Clinical Chemistry at the Hospitals of Malmö and Lund, Sweden.

An apparatus is described for paper electrophoresis according to the moist-chamber principle. A simple method for quantitative evaluation of the different electrophoretic fractions is presented together with the limits for the normal variation of the different serum fractions.

The influence of the composition of the buffer on the separation of the proteins is stressed.

Addition of small amounts of calcium ions results in a separation of the beta-fraction into two distinct fractions, beta1 and beta2Ca. The mobility of beta-lipoproteins is decreased in the presence of calcium. The phenomenon was confirmed with the aid of moving-boundary electrophoresis. The refractive contribution of the lipids in the beta-lipoproteins in moving-boundary electrophoresis is shown. The mobility of the main beta-component containing carbohydrate and of the transferrin is not changed by calciumion.

Submitted on June 14, 1955




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Copyright © 1956 by the American Association for Clinical Chemistry.