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Clinical Chemistry 2: 160-165, 1956;
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Clinical Chemistry, Vol 2, 160-165, Copyright © 1956 by the American Association for Clinical Chemistry

Azorubin in Aqueous and Methanolic Solutions

Spectrophotometric Studies

Howard S. Friedman 1

1 Tampa Municipal Hospital, Tampa, Fla.

A study of the spectrophotometric characteristics of azorubin in varying proportions of methanol and water has been presented.

The absorbence of azorubin in water was found to vary from 73 to 90 per cent of that in methanol, over a concentration range of 0.001 to 0.025 mg. per 2 ml. of test solution.

The absorbence of azorubin was found to be linear over the same range of concentration, and over the range from 0.1 to 10 mg. per 100 ml. of serum, using a final test dilution of 1 to 8.

The strong probability of the artefactual nature of "direct" and "total" serum bilirubin values has been demonstrated.

Submitted on August 11, 1955






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Copyright © 1956 by the American Association for Clinical Chemistry.