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Clinical Chemistry 2: 175-183, 1956;
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Clinical Chemistry, Vol 2, 175-183, Copyright © 1956 by the American Association for Clinical Chemistry

Serum Iron Determination

George R. Kingsley 1 and Gloria Getchell 1

1 Department of Physiological Chemistry, School of Medicine, University of California at Los Angeles, and the Clinical Biochemistry Laboratory, Veterans Administration Center, Los Angeles, Calif.

1. A sensitive, reproducible method for serum iron determination has been developed employing extraction of serum acid hydrolysate with isoamyl alcohol and development of a highly colored ferrous complex of 4,7-diphenyl-1, 1O-phenanthroline for quantitative photometric measurement of iron.

2. The method gives good recoveries and also good agreement with the dry-ashing procedure.

3. A normal range of 120-200 µg./100 ml. (average 160) for the female and 125-238 µg./100 ml. (average 182) for the male for serum iron was obtained by the method.

Submitted on September 27, 1955






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Copyright © 1956 by the American Association for Clinical Chemistry.