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Clinical Chemistry 2: 195-213, 1956;
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Clinical Chemistry, Vol 2, 195-213, Copyright © 1956 by the American Association for Clinical Chemistry

Quantitative Nephelometric Microdetermination of Alpha-Globulins in Serum and Cerebrospinal Fluid

Abraham Saifer 1 and Michael C. Zymaris 1

1 Biochemistry Department, Isaac Albert Research Institute, Jewish Chronic Disease Hospital, Brooklyn 3, N. Y.

1. A micronephelometric method is presented for the quantitative determination of total alpha-globulins in both serum and cerebrospinal fluid. The method is based on the relatively specific interaction of this protein fraction with the cationic detergent, Octab, at pH 6.65 in collidine-NaCl (.08M) buffer.

2. The various Cohn's protein fractions (Method 6) (25) were investigated with the nephelometric procedure. In comparison with normal serum, Fractions IV-6, IV-1, and IV-4, respectively showed the greatest detergent reactivity per milligram of alpha-globulin.

3. The variations of such factors as the concentrations of reagents, protein/detergent ratio, and salt concentration were reinvestigated.

4. Analysis of 14 normal and 33 pathologic sera gave excellent checks with the total alpha-globulin values obtained with the Tiselius electrophoretic method.

5. Application of the procedure to the analysis of the alpha-globulin content of cerebrospinal fluid required the addition of standard serum to 0.5-ml. aliquots of spinal fluid for stoichiometric results.

Submitted on June 17, 1955






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Copyright © 1956 by the American Association for Clinical Chemistry.