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Clinical Chemistry 2: 303-319, 1956;
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Clinical Chemistry, Vol 2, 303-319, Copyright © 1956 by the American Association for Clinical Chemistry

Quantitative Electrophoresis of Serum Proteins on Paper

Moses Wurm 1 and Frederick H. Epstein 1

1 Research Department, Sidney Hillman Health Center, New York, N. Y.

1. A procedure for paper electrophoresis has been described which gives highly reproducible protein patterns with good resolution and freedom from distortions.

2. Densitometry of protein bands on paper stained with bromphenol blue or Amidoschwarz 10B reveals that the logarithm of protein concentration is proportional to optical density and that Beer's law does not apply. Electrophoretic patterns of normal human serum evaluated in this manner give values in close agreement with those obtained by moving-boundary electrophoresis.

3. Confidence limits were determined for both methods.

Submitted on November 15, 1955




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Copyright © 1956 by the American Association for Clinical Chemistry.