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Clinical Chemistry 2: 320-327, 1956;
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Clinical Chemistry, Vol 2, 320-327, Copyright © 1956 by the American Association for Clinical Chemistry

Blood pH Measurement with the Glass Electrode

Study of Venous and Fingertip Blood

Samuel Natelson 1 and Norbert Tietz 1

1 Rockford Memorial Hospital, Rockford, Ill.

A study has been described to explain the tendency of fingertip blood from normal individuals to drift to alkaline pH more rapidly than the blood of venous serum at 37°. This occurs even under oil and without loss of CO2. Evidence is offered to indicate that this phenomenon is due to the release of carbonic anhydrase in greater concentrations into the serum when the finger is punctured than when venipuncture is performed. An explanation is offered for the fact that serum from patients in acidemia with low CO2. content will not drift at 37°.

It is suggested that blood pH determinations be made by collecting the specimen under oil, cooling rapidly to room temperature, and performing the determination under oil on the separated serum.

Submitted on November 22, 1955




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Copyright © 1956 by the American Association for Clinical Chemistry.