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Clinical Chemistry 2: 394-400, 1956;
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Clinical Chemistry, Vol 2, 394-400, Copyright © 1956 by the American Association for Clinical Chemistry

New Biuret Reagent for the Determination of Proteins in Cerebrospinal Fluid

Harold L. Rosenthal 1 and Helen I. Cundiff

1 Division of Biochemistry, Department of Pathology, The Rochester General Hospital, Rochester, N. Y.

A new biuret reagent is described which contains disodium ethylen diamine tetraacetate as the copper stabilizing agent. The reagent effectively prevents the formation of cloudiness in determinations of cerebrospinal fluid due, in part, to the precipitation of calcium in alkaline solution. Results obtained with the new reagent compare favorably with those obtained by Kjeldahls analysis.

Submitted on December 27, 1955






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Copyright © 1956 by the American Association for Clinical Chemistry.