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Clinical Chemistry 20: 15-18, 1974;
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Clinical Chemistry, Vol 20, 15-18, Copyright © 1974 by the American Association for Clinical Chemistry

Determination of Glucose with a Research Model Aminco "Rotochem" by the Hexokinase Reaction

William Hasson 1, James R. Penton 1, and Graham M. Widdowson 1

1 Institute of Health Research, 2200 Webster St., Room 235, San Francisco, Calif. 94115.

A method involving the hexokinase reaction is described for determining glucose in serum with a Research Model Aminco "Rotochem." The need to measure a separate sample blank is eliminated by using the computational capabilities of a small computer interfaced with the Rotochem. In this modification of the method of Tiffany et al., a cubic curve is fitted to the initial portion of the reaction path. Sample blanks are calculated by extrapolation back to zero time. The procedure is precise and results correlate well with those for an accepted automated procedure in which the same reaction is used.


Key Words: computer used in the clinical laboratory • enzymatic method • AutoAnalyzer • centrifugal analyzer • cubic curve used for extrapolation

Submitted on June 25, 1973
Accepted on October 16, 1973






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Copyright © 1974 by the American Association for Clinical Chemistry.