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Clinical Chemistry 20: 1295-1304, 1974;
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Clinical Chemistry, Vol 20, 1295-1304, Copyright © 1974 by the American Association for Clinical Chemistry

Evaluation of a Gilford "Automatic Enzyme Analyzer" Modified to Assay 60 Samples per Hour

Samuel A. Morell 1, Daniel A. Bach 1, and Valborg E. Ayers 1

1 Department of Biochemistry, Medical College of Wisconsin, Milwaukee, Wis. 53233.

A Gilford 3400 Automatic Enzyme Analyzer has been modified to assay 60 samples per hour in modes 1 and 2 (28 and 61 seconds of incubation) or 30 samples per hour in mode 3 (187 seconds of incubation). The calculation cycle was reduced threefold to print activity based on DgrA/20 s, rather than DgrA/60 s. Linear ranges at 30 and 37 °C for lactate dehydrogenase, aspartate aminotransferase, and alkaline phosphatase were determined with control sera at rates of 60 samples per hour and for creatine kinase activated by dithiothreitol at 30 samples per hour. AutoAnalyzer end-point assays of patients' sera were correlated with the Gilford U/liter values. Application of the Micromedic pipette to the Gilford AEA system provided a convenient mean to study the linear range, precision, and accuracy of an enzyme assay.


Key Words: enzyme activity in serum • AutoAnalyzer • Micromedic pipette, precision and accuracy

Submitted on June 3, 1974
Accepted on July 25, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.