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Clinical Chemistry 20: 1320-1323, 1974;
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Clinical Chemistry, Vol 20, 1320-1323, Copyright © 1974 by the American Association for Clinical Chemistry

Use of a Laser-equipped Centrifugal Analyzer for Kinetic Measurement of Serum IgG

Gregory J. Buffone 1, John Savory 1, and R. E. Cross 1

1 Division of Clinical Chemistry, Departments of Medicine, Pathology, Biochemistry, and Hospital Laboratories, The University of North Carolina at Chapel Hill, Chapel Hill, N. C. 27514.

Use of a visible laser in an Aminco "Rotochem" centrifugal analyzer enables light-scattering measurements to be made with this instrument. The modification is technically simple, inexpensive, and requires no fundamental changes in basic instrument design. The laser-modified analyzer has been applied to the measurement of IgG in serum. A two-point kinetic analysis is used to quantitate IgG from a standard curve: protein concentration vs. change in intensity of the scattered light during a fixed time interval. Fourteen 20-µl samples can be simultaneously determined in 1.9 min. No sample blank corrections are required. Within-run precision studies, based in each case on 56 replicate measurements, yielded coefficients of variation of 3.8% and 4.8% for normal and abnormal pools, respectively.


Key Words: AutoAnalyzer • continuous-flow method compared • "Rotochem" centrifugal analyzer • measurement of nanogram amounts of proteins

Submitted on June 10, 1974
Accepted on July 26, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.