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Clinical Chemistry 20: 1341-1343, 1974;
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Clinical Chemistry, Vol 20, 1341-1343, Copyright © 1974 by the American Association for Clinical Chemistry

Systematic Bias between Blood-pH Instruments

Jack H. Ladenson 1, Carl H. Smith 2, David N. Dietzler 2, and J. E. Davis 1

1 Division of Laboratory Medicine, Washington University School of Medicine and Barnes Hospital, St. Louis, Mo. 63110.
2 Department of Pediatrics, Washington University School of Medicine and St. Louis Children's Hospital, St. Louis, Mo. 63110.

Measurement of tris(hydroxymethyl)aminomethane buffers and blood samples was used to compare the following commercial pH instruments: Corning Models 160 and 165, Instrumentation Laboratory, Inc. Model 313, and Radiometer Models E5021 and BMS 3MK2. These comparisons uncovered a systematic bias of 0.02 pH units in Instrumentation Laboratory, Inc. electrodes manufactured before April 1973. Other factors found to affect analytical results were the temperature of the saturated KCl salt bridge and reference electrode, the design of the temperature-control system, and the buffer used to standardize the instruments.

Submitted on December 28, 1973
Accepted on August 2, 1974







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Copyright © 1974 by the American Association for Clinical Chemistry.