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Clinical Chemistry, Vol 20, 1362-1363, Copyright © 1974 by the American Association for Clinical Chemistry
1
1 Research Institute of Pure Chemicals, Lachema, N. C., 621 33
Brno; and Institute of Medical Chemistry, School of Medicine, J.
E. Purkyn
University, Brno, Czechoslovakia.
We studied the optimal composition of EDTA-chelated biuret reagent for the determination of protein in serum. A reagent containing 18 mmol of EDTA, 15 mmol of Cu2+, and 1 mol of NaOH per liter exhibits a very low blank but about the same sensitivity as tartrate-chelated reagents. Maximum color intensity is attained within 25 min at room temperature, and adheres to Beer's law up to 120 g of protein per liter.
Submitted on April 11, 1974
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