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Clinical Chemistry 20: 1397-1402, 1974;
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Clinical Chemistry, Vol 20, 1397-1402, Copyright © 1974 by the American Association for Clinical Chemistry

Separation and Quantitation of Coproporphyrin and Uroporphyrin in Urine

Charles Sobel 1, Carlos Cano 1, and Ralph E. Thiers 1

1 Department of Research, Bio-Science Laboratories, Van Nuys, Calif. 91405.

Coproporphyrin and uroporphyrin are separated from urine and from each other by absorption onto, and selective elution from, an anion-exchange resin. The two compounds are separated by using solvents differing in both polarity and pH. The porphyrins are then quantitated with an Aminco-Bowman Spectrophotofluorometer equipped with an ellipsoidal mirror and a red-sensitive photomultiplier tube. Porphyrins can be determined in 1 ml of urine from a normal individual.


Key Words: anion-exchange chromatography • porphyrinuria • normal values • fluorometry • porphyrin stability

Submitted on May 23, 1974
Accepted on July 25, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.