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Clinical Chemistry 20: 1543-1547, 1974;
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Clinical Chemistry, Vol 20, 1543-1547, Copyright © 1974 by the American Association for Clinical Chemistry

Gas—Liquid Chromatography of Phenylalanine Metabolites in Urine and Sera of Hyperphenylalaninemic and Phenylketonuric Patients

Teruo Kitagawa 1

1 Department of Pediatrics, Waisman Center on Mental Retardation, 2605 Marsh Lane, University of Wisconsin Medical Center, Madison, Wis. 53706.

Gas—liquid chromatographic methods are described for measuring phenylalanine metabolites in urine and serum. These were studied in controls and in various types of hyperphenylalaninemics. The plasma phenyllactic acid concentration increased as the serum phenylalanine concentration increased. The 24-h urinary excretion of both phenylalanine and phenyllactic acid depends upon their respective concentrations in serum.


Key Words: phenyllactic acid, phenylpyruvic, and o- and p-hydroxyphenylacetic acid • methylester derivatives • phenylalanine and phenyllactic acid concentrations in serum, and relation to urinary excretion

Submitted on March 22, 1971






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Copyright © 1974 by the American Association for Clinical Chemistry.