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Clinical Chemistry 20: 1564-1567, 1974;
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Clinical Chemistry, Vol 20, 1564-1567, Copyright © 1974 by the American Association for Clinical Chemistry

Standardization of Serum Ceruloplasmin Concentrations in International Enzyme Units with o-Dianisidine Dihydrochloride as Substrate

H. Peter Lehmann 1, Karl H. Schosinsky 1, and Myrton F. Beeler 1

1 Department of Pathology, Louisiana State University Medical Center, New Orleans, La. 70112.

We describe a method for calculating the absorptivity (in terms of substrate consumed) of the colored solution obtained when o-dianisidine dihydrochloride is oxidized by ceruloplasmin. By oxidizing o-dianisidine dihydrochloride with known amounts of hydrogen peroxide we could determine that the molar reacting ratio of o-dianisidine to hydrogen peroxide is 2:1. So calculated, absorptivity is 9.6 ml µmol-1 cm-1, the figure used to estimate ceruloplasmin oxidase activity in terms of International Units.


Key Words: enzyme activity • normal values

Submitted on June 13, 1974
Accepted on October 5, 1974




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