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Clinical Chemistry 20: 159-162, 1974;
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Clinical Chemistry, Vol 20, 159-162, Copyright © 1974 by the American Association for Clinical Chemistry

Spectrophotometric Determination of Ethchlorvynol in Biologic Specimens

Jack E. Wallace 1, Horace E. Hamilton 1, Joel A. Riloff 1, and Kenneth Blum 1

1 Department of Pathology, The University of Texas Medical School at San Antonio, San Antonio, Tex. 78829 (J.E.W. and H.E.H.); the Biochemistry Branch, USAF School of Aerospace Medicine, Brooks AFB, Texas (J.A.R.); and Department of Pharmacology, The University of Texas Medical School at San Antonio (K.B.).

A sensitive, highly specific spectrophotometric method for quantitative determination of ethchlorvynol in biologic specimens is described. The procedure is based on conversion of ethchlorvynol to a product mixture consisting of two major components that strongly absorb ultraviolet light. Results can be obtained within 45 min of receipt of a specimen.


Key Words: spectrophotometry • concentrations of ethychlorvynol in blood • drug derivatization for analysis

Submitted on May 31, 1973
Accepted on October 1, 1973






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Copyright © 1974 by the American Association for Clinical Chemistry.