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Clinical Chemistry 20: 278-281, 1974;
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Clinical Chemistry, Vol 20, 278-281, Copyright © 1974 by the American Association for Clinical Chemistry

New Method for Rapid Determination of Carboxyhemoglobin by Use of Double-Wavelength Spectrophotometry

Alfred Ramieri Jr. 1, Peter Jatlow 1, and David Seligson 1

1 Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Conn. 06510.

We have developed a simple method for rapid determination of carboxyhemoglobin concentration in blood, by use of double-wavelength spectrophotometry. The absorbance of both oxyhemoglobin and reduced hemoglobin are instrumentally nulled out in this procedure, so that the absorption signal reflects HbCO and only a single spectrophotometric reading is required. This procedure is accurate in the range encountered in ambulatory persons as well as in the "toxic" range. Results correlated well with the dithionite reduction procedure. The stability of carboxyhemoglobin in solution was investigated and concentrations of carbon monoxide in groups of smokers and nonsmokers were determined.


Key Words: comparison with dithionite method • CO in blood of smokers • CO poisoning • toxicology

Submitted on November 15, 1973
Accepted on November 26, 1973






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Copyright © 1974 by the American Association for Clinical Chemistry.