Clinical Chemistry, Vol 20, 348-352, Copyright © 1974 by the American Association for Clinical Chemistry

Automated Simultaneous Determination of p-Aminohippurate and Creatinine in Plasma or Urine

¹ Department of Toxicology, The Squibb Institute for Medical Research, New Brunswick, N. J. 08903.

Methods have been developed for using the Beckman "Discrete Sample Analyzer" (DSA-560), for automated simultaneous determinations of p-aminohippurate (PAH) and creatinine in the same 50- or 10-µl samples of plasma or urine, respectively, at the rate of 80 samples per hour. In determinations of PAH, a single reagent, p-dimethylaminocinnamaldehyde in 0.1 mol/liter HCl, was added to a protein-free filtrate from plasma or urine. The intensity of the color, measured at 550 nm, obeyed Beer’s law for PAH concentrations from 0.005 to 5.0 g/liter. Data obtained for the same plasma and urine samples by the manual method, were, in general, within ±5% of the results obtained by the automated method. Creatinine was simultaneously determined by adapting the method of Taussky to the DSA-560 instrument; the intensity of the color, measured at 510 nm, obeyed Beer's law for creatinine concentrations ranging from 0.005 to 5.0 g/liter. Data obtained for the same plasma and urine samples by the manual method, were, in general, within ± 5% of the results obtained by the automated method.

Submitted on April 25, 1973
Accepted on December 14, 1973

The following articles in journals at HighWire Press have cited this article:

				R. Agarwal Rapid microplate method for PAH estimation Am J Physiol Renal Physiol, August 1, 2002; 283(2): F236 - F241. [Abstract] [Full Text] [PDF]