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Clinical Chemistry 20: 353-359, 1974;
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Clinical Chemistry, Vol 20, 353-359, Copyright © 1974 by the American Association for Clinical Chemistry

Use of Enzyme-Linked Antibodies to Measure Serum Anti-DNA Antibody in Systemic Lupus Erythematosus

A. J. Pesce 1, N. Mendoza 1, I. Boreisha 1, M. A. Gaizutis 1, and V. E. Pollak 1

1 Division of Nephrology, Department of Medicine, University of Cincinnati Medical Center, Cincinnati, Ohio 45229; and the Renal Division, Michael Reese Hospital and Medical Center, Chicago, Ill. 60616.

We describe a solid-phase adsorbent enzyme-linked immunoassay for measuring the amount of antibody in serum. The assay consists of adsorbing an excess of DNA to a polystyrene test-tube support, reacting the anti-DNA serum with the coated tube, following with an additional reaction of an anti-human ggr-globulin peroxidase enzyme conjugate with the coated tube, and finally developing a colored reaction product by the action of peroxidase on substrate. In principle, the amount of IgG bound to the tube is proportional to the amount of anti-DNA contained in the serum, and the conjugate also reacts proportionally to the amount of human IgG that has reacted. The assay gives data consistent with that expected for serum from patients with systemic lupus erythematosus, and it is possible to titrate the serum for micrograms of DNA antibody. At present the method still has considerable variability, but offers a new approach to measurement of antibody in autoimmune diseases.


Key Words: horse-radish peroxidase—antibody complex • antibody measurement in autoimmune disease • immunologic disorders • immunoassay • solid-phase adsorbent • immunohistologic method compared

Submitted on October 24, 1973
Accepted on December 14, 1973




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[Abstract] [Full Text] [PDF]


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