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Clinical Chemistry 20: 389-391, 1974;
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Clinical Chemistry, Vol 20, 389-391, Copyright © 1974 by the American Association for Clinical Chemistry

Shortened Radioimmunoassay for Human Growth Hormone

S. L. Twomey 1, J. M. Beattie 1, and G. T. Wu 1

1 Bio-Science Laboratories, 7600 Tyrone Ave., Van Nuys, Calif. 91405.

We report a radioimmunoassay procedure for human growth hormone in serum, in which the assay mixture is incubated at 37 °C for a total of 5 h rather than at 4 °C for 48 h. There was no significant difference in results by the two methods, according to the Sign Test and the signed-rank statistical test of Wilcoxin. A comparison of results at the two temperatures demonstrated a Spearmann coefficient of rank correlation value of 0.94. With this facilitation, a laboratory can provide results on the same day that the sample arrives.


Key Words: double antibody • statistical treatment of data

Submitted on November 6, 1973
Accepted on December 18, 1973






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Copyright © 1974 by the American Association for Clinical Chemistry.