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Clinical Chemistry 20: 394-395, 1974;
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Clinical Chemistry, Vol 20, 394-395, Copyright © 1974 by the American Association for Clinical Chemistry

Dual-Precipitation Method for Lipoprotein Quantitation: Improvement by Membrane Ultrafiltration of Lipemic Samples

Dana E. Wilson 1 and Gregory A. Done 1

1 Department of Medicine, University of Utah College of Medicine, Salt Lake City, Utah 84132.

Plasma lipoproteins can be measured by the clinical laboratory after the major lipoprotein classes have been separated by two precipitation steps, one of which is the aggregation of very-low-density lipoproteins (VLDL) by the detergent, sodium dodecyl sulfate. Lipoprotein concentrations are expressed in terms of the cholesterol concentration within each class. However, incomplete removal of aggregated VLDL has led to serious analytical errors in a few lipemic subjects. We tested the efficacy of a membrane ultrafiltration step in removal of aggregated VLDL in three patients with plasma triglyceride concentrations of 540 to 12 560 mg/dl. Preliminary membrane ultrafiltration to remove the aggregated VLDL improved the precision of the method and is recommended for the analysis of grossly lipemic samples.

Submitted on November 12, 1973
Accepted on December 19, 1973






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Copyright © 1974 by the American Association for Clinical Chemistry.