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Clinical Chemistry 20: 421-423, 1974;
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Clinical Chemistry, Vol 20, 421-423, Copyright © 1974 by the American Association for Clinical Chemistry

Column-Chromatographic Analysis of Naturally Fluorescing Compounds: III. Rapid Analysis of Serotonin and Tryptamine

D. D. Chilcote 1

1 Oak Ridge National Laboratory,2 Oak Ridge, Tenn. 37830.

A previously described [Clin. Chem. 18, 778 (1972)] chromatographic separation and fluorimetric detection system has been adapted to the determination of serotonin and tryptamine. A strong cation-exchange resin, "Aminex A-6," is used. At a column temperature of 60 °C, serotonin and tryptamine are eluted in 0.5 and 1.0 h, respectively, with an ammonium acetate-acetic acid buffer solution (6 mol of acetate per liter). The sensitivity of the detector allows reliable quantitation of 100 ng of each amine in the sample loaded onto the column.


Key Words: fluorimetry • neurohumoral agent • diagnosis of malignant carcinoid, Hartnup’s disease

Submitted on November 28, 1973
Accepted on January 11, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.