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Clinical Chemistry 20: 494-496, 1974;
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Clinical Chemistry, Vol 20, 494-496, Copyright © 1974 by the American Association for Clinical Chemistry

Optimal agr-Oxobutyrate Concentration for Assay of agr-Hydroxybutyrate Dehydrogenase Activity in Normal and Pathological Sera at 30 °C

Leslie M. Shaw 1 and Jane Gray 1

1 The William Pepper Laboratory, Department of Pathology, University of Pennsylvania, School of Medicine, Philadelphia, Pa. 19104.

Optimal concentration of substrate, agr-oxobutyrate, for determination of agr-hydroxybutyrate dehydrogenase (HBD) activity in normal and pathological sera at 30 °C (with a centrifugal analyzer) is 12 mmol/ liter. With this substrate concentration, HBD activity averaged 146% of that found with the suboptimal concentration, 3.3 mmol/liter, usually used. Furthermore, day-to-day precision was significantly better. HBD/LD ranges (LD, lactate dehydrogenase), determined for sera from patients with myocardial infarction and liver disease, did not overlap and were similar to those established by Elliot et al. [Clin. Sci. 23, 305 (1962)], who used a suboptimal agr-oxobutyrate concentration of 3.3 mmol/liter at 25 °C. At 30 °C the mean HBD/LD ratios for sera from patients with myocardial infarction were most different from those for patients with liver disease when the optimal concentration of agr-oxobutyrate was used.


Key Words: centrifugal analyzer • Centrifi-Chem • normal values • diagnosis of liver disease, myocardial infarction

Submitted on October 17, 1973
Accepted on January 11, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.