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Clinical Chemistry 20: 544-546, 1974;
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Clinical Chemistry, Vol 20, 544-546, Copyright © 1974 by the American Association for Clinical Chemistry

Specific Automated Method for Measurement of Urinary Hydroxyproline

G. C. Seymour 1 and M. J. Jackson 1

1 Division of Clinical Chemistry. Northwick Park Hospital and Clinical Research Centre, Harrow, Middlesex HA1 3UJ, England.

An automated continuous-flow method is described for estimating total hydroxyproline in urine. The method utilizes column chromatography on sulfonated polystyrene resin, the eluate being reacted with chloramine-T and p-dimethylaminobenzaldehyde to produce a colored complex that is measured at 560 nm. The method is sensitive and specific, and requires 50 min per analysis.


Key Words: column chromatography • normal values • p-dimethylaminobenzaldehyde as chromogen • AutoAnalyzer • collagen metabolism

Submitted on January 10, 1974
Accepted on February 19, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.