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Clinical Chemistry 20: 547-552, 1974;
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Clinical Chemistry, Vol 20, 547-552, Copyright © 1974 by the American Association for Clinical Chemistry

Column-Chromatographic Studies of Isoamylases in Human Serum, Urine, and Milk

Louis Fridhandler 1, J. Edward Berk 1, Katherine A. Montgomery 1, and Deborah Wong 1

1 Department of Medicine, College of Medicine, University of California, Irvine, Calif.

Pancreatic and salivary isoamylases not discerned by a chromatographic method previously described have been detected by a modification of that method. Detailed analysis of the nature of these isoamylases and their distribution showed that the pancreatic type (P-type) and salivary type (S-type) isoamylases in pancreatic extract and saliva appear in normal urine along with an isoamylase of unknown origin (X-type). Normal serum could not be similarly studied because of the low amylase activity in such serum. However, the X-type isoamylase fraction was found in three hyperamylasemic sera, including one from a patient with lung carcinoma who produced S-type amylase. Study of 25 samples of human milk revealed high amylase activity and no detectable P-type; S-type components were found along with the X-type component. Parallel patterns of variation were found in human milk in two patients studied for six months postpartum. Salivary amylase and the S-type amylase in milk and in the serum of the patient with lung carcinoma and marked hyperamylasemia appeared to be similar, and may be identical.


Key Words: isoenzymes • amylasemia • Sephadex and DEAE-Sephadex chromatography • lung carcinoma • pancreatic and salivary isoamylases • nomenclature of isoamylases

Submitted on January 18, 1974
Accepted on February 18, 1974




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Copyright © 1974 by the American Association for Clinical Chemistry.