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Clinical Chemistry 20: 560-565, 1974;
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Clinical Chemistry, Vol 20, 560-565, Copyright © 1974 by the American Association for Clinical Chemistry

Automated Fluorometric Creatine Kinase Assay. Measurement of 100-Fold Normal Activity without Serum Dilution

J. B. Armstrong 1, J. A. Lowden 1, and A. L. Sherwin 2

1 The Hospital for Sick Children, Toronto, Canada.
2 The Montreal Neurological Hospital, Montreal, Canada.

Creatine kinase (EC 2.7.3.2) activity can be measured fluorometrically by coupling the reaction product, creatine, to ninhydrin at alkaline pH. We have automated this procedure to develop an accurate and precise method, which requires 0.1 ml of sample and can be performed at 60 tests per hour. By using an abbreviated incubation time and a dialyzer in the assay, we have extended the analytical range to 100 times normal serum activity without sample dilution. The dialyzer also eliminates the nonspecific protein fluorescence of the sample. An additional advantage of the method is the reduced cost of reagents in comparison to similar procedures. Serum blanks should be analyzed whenever serum creatine is abnormally high, especially in cases of severe renal failure.


Key Words: renal failure • ninhydrin reaction • interference from creatine • normal values

Submitted on July 11, 1973
Accepted on February 19, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.