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Clinical Chemistry 20: 582-585, 1974;
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Clinical Chemistry, Vol 20, 582-585, Copyright © 1974 by the American Association for Clinical Chemistry

Comparative Study of a Micro-Scale Test for Lead in Blood, for Use in Mass Screening Programs

Richard E. Cooke 1, Kathryn L. Glynn 1, William W. Ullmann 1, Nicole Lurie 2, and Martha Lepow 2

1 Connecticut State Department of Health, Laboratory Division, 10 Clinton St., Hartford, Conn. 06106.
2 Department of Pediatrics, University of Connecticut School of Medicine, Farmington Ave., Farmington, Conn. 06032.

A procedure suitable for the mass screening of children for an undue body burden of lead was tested on 170 clinic patients. Capillary finger-prick blood, collected on filter paper, was analyzed for lead with an atomic absorption spectrophotometer with the Delves cup attachment. This method of analysis had first been judged to be accurate after a laboratory evaluation in which results were compared with those obtained by a dithizone procedure. In the present study, both capillary and venous bloods were obtained from the patients. Venous bloods were also analyzed by the filter paper disk method and by an atomic absorption spectrophotometric procedure requiring a 5-ml sample. Results show that the screening method described here will reliably identify those children with a significantly supranormal blood lead. Both sample collection and laboratory analysis are easier.


Key Words: Delves-cup flame-spectrophotometric technique • trace metals • contamination problems • toxicology • dithizone procedure compared • atomic absorption spectrophotometry • sources of variation

Submitted on March 22, 1973
Accepted on February 28, 1974




The following articles in journals at HighWire Press have cited this article:


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N. V. Stanton, J. M. Maney, and R. Jones
Evaluation of Filter Paper Blood Lead Methods: Results of a Pilot Proficiency Testing Program
Clin. Chem., December 1, 1999; 45(12): 2229 - 2235.
[Abstract] [Full Text] [PDF]


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Copyright © 1974 by the American Association for Clinical Chemistry.