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Clinical Chemistry 20: 617-619, 1974;
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Clinical Chemistry, Vol 20, 617-619, Copyright © 1974 by the American Association for Clinical Chemistry

Improved Automated Method for Determining Serum Albumin with Bromcresol Green

Porntip H. Lolekha 1 and Wasana Charoenpol 1

1 Department of Clinical Pathology, Ramathibodi Hospital, Mahidol University, Rama VI Road, Bangkok 4, Thailand.

An automated method for determining serum albumin with bromcresol green according to Doumas et al. [Clin. Chim. Acta 31, 87 (1971)] was simplified and improved for use in routine analysis. The manifold is simpler, there is better sample separation and less sample interaction, both within-run and day-to-day run precision are excellent, and results agree well with those obtained by electrophoresis on cellulose acetate.


Key Words: electrophoresis on cellulose acetate compared • total protein in serum determined simultaneously

Submitted on November 28, 1973
Accepted on February 26, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.