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Clinical Chemistry 21: 109-112, 1975;
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Clinical Chemistry, Vol 21, 109-112, Copyright © 1975 by the American Association for Clinical Chemistry

Thin-layer Chromatography and Enzyme Immunoassay of L-Alpha-Acetyl Methadol and Methadone Metabolites in Urine

Joyce A. McIntyre 1, Arlene E. Armandi 1, Lance P. Risen 1, Walter Ling 1, and George C. Haberfeldel 1

1 Department of Pathology, Veterans Administration Hospital, Sepulveda, Calif. 91343 and the Department of Psychiatry, University of California at Los Angeles, Los Angeles, Calif. 90024.

We describe a thin-layer chromatographic technique suitable for isolating at least four metabolites of L-agr-acetyl methadol. The metabolites are discussed in terms of their color reactions to chromatography spray reagents and their appearance in the urine during various time intervals after dosage. Methadone is detected by the presence of the unmetabolized drug and two of its metabolites in the urine. We present a simple means of distinguishing methadone-containing specimens from those that contain L-agr-acetyl methadol. Results of an immunoassay for methadone applied to specimens from patients receiving L-agr-acetyl methadol or methadone are discussed. The method is suitable as a routine screening procedure for most laboratories that use thin-layer chromatography.


Key Words: toxicology • screening • drug abuse

Submitted on May 20, 1974
Accepted on October 18, 1974






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Copyright © 1975 by the American Association for Clinical Chemistry.