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Clinical Chemistry 21: 113-118, 1975;
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Clinical Chemistry, Vol 21, 113-118, Copyright © 1975 by the American Association for Clinical Chemistry

Continuous-Flow Measurement of Lactate in Blood: A Technique Adapted for Use in the Emergency Laboratory

Edward Boycks 1, Sybil Michaels 1, Max Harry Weil 1, Herbert Shubin 1, and Edward P. Marbach 1

1 The Shock Research Unit and the Department of Medicine, University of Southern California School of Medicine, the Los Angeles County/USC Medical Center, and the Center for the Critically Ill, Hollywood Presbyterian Medical Center, Los Angeles, Calif. 90027.

Max Harry Weil, M.D., University of Southern California School of Medicine, Center for the Critically Ill, 1300 N. Vermont Ave., Los Angeles, Calif. 90027.

This automated enzymatic method for analysis of lactate in blood, adapted from a manual method, is designed for use with an AutoAnalyzer. Start-up time is 5 min. After a time delay of 4 min, 99 samples may be analyzed per hour. Recovery averaged 100.7% (range, 99.6-101.8%). Reproducibility was within 1 SD (equivalent to 70 µmol/liter). Reagents are stable at room temperature for one month. The procedure has been in continuous use as an emergency test in support of emergency and critical care for three years, during which time we have done more than 10 000 determinations.


Key Words: monitoring the severely ill • acute circulatory failure

Submitted on August 24, 1974
Accepted on October 18, 1974






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Copyright © 1975 by the American Association for Clinical Chemistry.