Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 21: 119-124, 1975;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Blaedel, W. J.
Right arrow Articles by Uhl, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Blaedel, W. J.
Right arrow Articles by Uhl, J. M.

Clinical Chemistry, Vol 21, 119-124, Copyright © 1975 by the American Association for Clinical Chemistry

Nature of Materials in Serum That Interfere in the Glucose Oxidase—Peroxidase—o-Dianisidine Method for Glucose, and Their Mode of Action

Walter J. Blaedel 1 and James M. Uhl 1

1 Department of Chemistry, University of Wisconsin, Madison, Wis. 53706.

Separation of blood serum on Sephadex G-100 reveals three fractions that interfere with the glucose oxidase— peroxidase method for serum glucose when o-dianisidine is used as the chromogen. A low-molecular-weight fraction containing primarily uric acid, a fraction containing protein with a molecular weight of about 40 000, and a fraction of even higher molecular weight (sim 500 000) each interfered with glucose recovery when glucose was measured by this procedure. The uric acid fraction and the isolated 40 000 molecular weight fraction interfere by competing with o-dianisidine for hydrogen peroxide in the peroxidase-catalyzed color-formation step. The high-molecular-weight fraction not only interferes with the peroxidase reaction, but also with the glucose oxidase reaction itself. These agents cause values to be low by as much as 20% in the manual determination of glucose in normal serum if their interference is not recognized.


Key Words: interference by uric acid and high-molecular-weight materials • interfering material in urine

Submitted on July 25, 1974
Accepted on October 21, 1974






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1975 by the American Association for Clinical Chemistry.