Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 21: 144-147, 1975;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Johnson, G. F.
Right arrow Articles by Solomon, H. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Johnson, G. F.
Right arrow Articles by Solomon, H. M.

Clinical Chemistry, Vol 21, 144-147, Copyright © 1975 by the American Association for Clinical Chemistry

Gas-Chromatographic Determination of Theophylline in Human Serum and Saliva

George F. Johnson 1, Walter A. Dechtiaruk 1, and Harvey M. Solomon 1

1 Department of Laboratory Medicine, The Johns Hopkins Hospital, Baltimore, Md. 21205.

We report a gas-chromatographic procedure for routine determination of the bronchodilator, theophylline (1,3-dimethylxanthine), in serum and saliva. Volatile butyl derivatives for gas chromatography were prepared by the method of Greeley [Clln. Chem. 20, 192 (1974)], with butyliodide as the alkylating agent. Substances that interfere in the classical determination of theophylline, such as theobromine (3,7-dimethylxanthine) and phenobarbital, are well separated from theophylline by the gas-chromatographic procedure and can be quantitated if desired. It is possible to determine 1 µg of theophylline in 1 ml of serum or saliva. Between-run precision for 15 µg/ml samples was 5.3%. This determination is useful in assessing patient compliance with medication instructions and in adjusting dosage schedules of theophylline in patients attending a pediatric allergy clinic.


Key Words: measurement of theobromine, phenobarbital • monitoring of therapy • toxicology

Accepted on October 30, 1974




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
R. Rej
Clinical Chemistry through Clinical Chemistry: A Journal Timeline
Clin. Chem., December 1, 2004; 50(12): 2415 - 2458.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1975 by the American Association for Clinical Chemistry.