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Clinical Chemistry 21: 148-150, 1975;
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Clinical Chemistry, Vol 21, 148-150, Copyright © 1975 by the American Association for Clinical Chemistry

Solid-Phase Radioimmunoassay on Polystyrene Beads and Its Application to Dopamine-beta-Hydroxylase

Robert A. Rush 1, Shmuel H. Kindler 1, and Sidney Udenfriend 1

1 Roche Institute of Molecular Biology, Nutley, N. J. 07110.

Antibody-coated polystyrene beads have been used to develop a solid-phase radioimmunoassay for bovine dopamine-beta-hydroxylase (EC 1.14.17.1). The technique is simple and offers many advantages over other radioimmunoassay techniques, including solid-phase procedures, a major one being its adaptability to a wide range of antigen concentrations. Precision, sensitivity, and reproducibility compare favorably with those of existing methods. The ease and speed with which this test is performed have made it especially useful for clinical evaluations of serum dopamine-beta-hydroxylase. Antibody-coated beads should be applicable for the assay of other antigens.

Submitted on August 28, 1974
Accepted on October 7, 1974






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Copyright © 1975 by the American Association for Clinical Chemistry.