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Clinical Chemistry 21: 67-70, 1975;
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Clinical Chemistry, Vol 21, 67-70, Copyright © 1975 by the American Association for Clinical Chemistry

Enzymatic Determination of Serum Urea on the Surface of Silicone-Rubber Pads

Jui-chang W. Kuan 1, Herbert K. Y. Lau 1, and George G. Guilbault 1

1 Department of Chemistry, University of New Orleans, New Orleans, La. 70122.

An enzymatic, fluorometric method is described for determination of serum urea on silicone-rubber pads. In this method, the reagents are lyophilized on the surface of the pads, NADH on one side and a mixture of urease, glutamate dehydrogenase, and agr-ketoglutarate on the other. The rate of disappearance of NADH fluorescence at 460 nm (excitation wavelength, 340 nm) is monitored and related to serum urea concentration. The calibration curve is linear to 250 mg of urea per liter. The method affords a rapid, simple, and inexpensive means for urea assay, the results of which correlate well with automatic diacetyl monoxime method (correlation coefficient, 0.998).

Submitted on June 12, 1974
Accepted on October 7, 1974






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Copyright © 1975 by the American Association for Clinical Chemistry.