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Clinical Chemistry 21: 1430-1436, 1975;
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Clinical Chemistry, Vol 21, 1430-1436, Copyright © 1975 by the American Association for Clinical Chemistry

New System for Automated Extraction and Simultaneous Determination of Serum Cholesterol and Triglycerides

Dorothy F. Wease 1, Eli S. Espinosa 1, and Yvonne J. Anderson 1

1 Clinical Pathology Branch, Clinical Sciences Division, USAF School of Aerospace Medicine, Aerospace Medical Division, AFSC, United States Air Force, Brooks AFB, Texas.

We describe an automated system for serum lipid extraction and simultaneous determination of cholesterol and triglycerides, with use of continuous-flow equipment. A sample volume of 100 µl of serum is required, and samples are processed at the rate of 20 per hour, which may be increased with slight loss in precision. Time from sample pickup to recorder readout is about 25 min. The system makes use of established colorimetric reactions, and the values obtained agree with ranges currently reported in the literature. Correlation coefficients for results of the automated and manual methods were 0.98 for cholesterol and 0.99 for triglycerides, and the day-to-day coefficients of variation were 1.8% for cholesterol (1 SD = 34 mg/liter) and 3.4% for triglycerides (1 SD = 37 mg/liter). The small sample volume, precision, accuracy, speed, and comparative economy of reagents make this system particularly suitable for multiphasic screening, pediatrics, and small-animal research.


Key Words: screening • pediatric chemistry • continuous-flow analysis • intermethod comparison

Submitted on February 24, 1975
Accepted on May 26, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.