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Clinical Chemistry 21: 1465-1468, 1975;
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Clinical Chemistry, Vol 21, 1465-1468, Copyright © 1975 by the American Association for Clinical Chemistry

Automated Immunochemical Method for Determination of Urinary Protein of Plasma Origin

Lawrence M. Killingsworth 1, Carol E. Britain 1, and Linda L. Woodard 1

1 Departments of Hospital Laboratories, Medicine, and Pathology, University of North Carolina School of Medicine, Chapel Hill, N. C. 27514.

Clinical Chemistry Laboratories, N. C. Memorial Hospital, Chapel Hill, N. C. 27514.

An automated continuous-flow procedure has been developed for the rapid determination of urinary proteins of plasma origin. Antiserum to whole human plasma was used as the reagent, and the antigen—antibody reactions were quantitated by nephelometry. By adding polyethylene glycol (mol wt 6000-7500) to the reaction medium, reaction time was decreased to <3 min; no sample blanks were required; and samples were analyzed at a rate of 70 per hour. Recovery studies yielded an average of 98.5% of the added protein. In-run replicate precision (CV) of the method was 1.45%; day-to-day precision was 2.58%.


Key Words: nephelometry • immunochemistry • continuous-flow analysis • kidney disease • normal (reference) values

Submitted on April 3, 1975
Accepted on June 19, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.