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Clinical Chemistry 21: 1493-1497, 1975;
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Clinical Chemistry, Vol 21, 1493-1497, Copyright © 1975 by the American Association for Clinical Chemistry

Centrifugal Analyzer Determination of Ascorbate in Serum or Urine with Fe3+/Ferrozine

William C. Butts 1 and Harold J. Mulvihill 1

1 Institute of Health Research, P. O. Box 7999, San Francisco, Calif. 94120.

Measurement of serum ascorbate may be useful in long-term population studies because of the possible influence of ascorbate on numerous physiological factors. We describe an automated method for determining ascorbate in serum and urine by using the reduction of ferric iron by ascorbate and the formation of a color between the resulting ferrous iron and Ferrozine [3-(2-pyridyl)-5,6-bis(4-phenylsulfonic acid)-1,2,4-triazine]. A centrifugal analyzer is used to rapidly and simultaneously measure ascorbate in the samples and standards and minimize interference from slower reacting substances in the sample. The method is highly precise and specific. Data are also presented on the stability of ascorbate in serum, urine, and aqueous solutions.


Key Words: stability of ascorbate in serum, urine, aqueous solutions • screening • analytical error • normal (reference) values

Submitted on April 17, 1975
Accepted on June 23, 1975




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Copyright © 1975 by the American Association for Clinical Chemistry.