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Clinical Chemistry 21: 1513-1514, 1975;
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Clinical Chemistry, Vol 21, 1513-1514, Copyright © 1975 by the American Association for Clinical Chemistry

Evaluation of an Automated Glucose-Oxidase Procedure

Jose A. Rodriguez-Castellon 1, C. Andrew Robinson 1, Mary S. Smith 1, and Joyce H. Frye 1

1 Department of Clinical Pathology, Veterans Administration Hospital; and University of Alabama in Birmingham, Birmingham, Ala. 35233.

We evaluated a slight modification of the automated oxidase/peroxidase method for glucose determination in blood with use of 4-aminophenazone [J. Clin. Pathol. 22, 246 (1969)]. Nonsugar reducing substances did not interfere, except for ascorbic acid in very high concentrations. Lipemic sera gave slightly lower values. Values for reproducibility, specificity, and sensitivity were closer to such values for the hexokinase method than was the case for some other commonly used methods.


Key Words: intermethod comparison

Submitted on March 20, 1975
Accepted on June 9, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.