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Clinical Chemistry 21: 1638-1643, 1975;
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Clinical Chemistry, Vol 21, 1638-1643, Copyright © 1975 by the American Association for Clinical Chemistry

Fluorometric Determination of "Albumin-Titratable Bilirubin" in the Jaundiced Neonate

Susan B. McCluskey 1, G. N. B. Storey 1, G. K. Brown 1, D. G. More 1, and W. J. O’Sullivan 1

1 Department of Paediatrics, Royal Prince Alfred Hospital, Camperdown, N.S.W. 2050, Australia; and Department of Medicine, University of Sydney, Sydney, N.S.W. 2006, Australia

Dr. W. J. O’S., School of Biochemistry, The University of New South Wales, P.O. Box 1, Kensington, N.S.W. 2033, Australia

We report a fluorometric technique for determination of albumin-titratable bilirubin in the jaundiced neonate. Although bilirubin alone has very little native fluorescence, considerable emission is observed in the presence of albumin under acid conditions. Analysis of the plasma sample alone and in the presence of excess human serum albumin solution appears to reflect the bilirubin tightly bound to albumin and the total serum bilirubin, respectively. The difference between these two values has been designated as "albumin-titratable bilirubin." Where the concentration of albumin-titratable bilirubin is considerable, a typical saturation effect is observed. In samples where the circulating bilirubin is strongly bound to endogenous albumin, no change in fluorescence is seen when exogenous albumin is added. Results correlate well with the clinical picture


Key Words: pediatric chemistry • liver disease diagnostic aid • kernicterus

Submitted on May 14, 1973
Accepted on July 25, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.