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Clinical Chemistry 21: 1721-1724, 1975;
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Clinical Chemistry, Vol 21, 1721-1724, Copyright © 1975 by the American Association for Clinical Chemistry

More Sensitive Automated Detection of Polyamines in Physiological Fluids and Tissue Extracts with o-Phthalaldehyde

Laurence J. Marton 1 and Patrick L. Y. Lee 2

1 Naffziger Laboratories for Neurosurgical Research, Department of Neurological Surgery, University of California, San Francisco, Calif. 94143.Department of Laboratory Medicine, University of California, San Francisco, Calif. 94143.
2 Durrum Instrument Corp., Palo Alto, Calif. 94303.

o-Phthalaldehyde reacts with primary amines to form an intensely blue-fluorescing product. Replacing ninhydrin with this compound in our automated high-pressure liquid-chromatographic technique for determination of polyamines, we achieved a 6-to 10-fold increase in sensitivity. Linearity and reproducibility are excellent, and the method is shown to be applicable to analysis of physiological fluids and of tissue extracts.


Key Words: cancer • high-pressure liquid chromatography

Submitted on July 7, 1975
Accepted on August 6, 1975




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[Abstract] [PDF]


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Copyright © 1975 by the American Association for Clinical Chemistry.