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Clinical Chemistry 21: 1747-1753, 1975;
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Clinical Chemistry, Vol 21, 1747-1753, Copyright © 1975 by the American Association for Clinical Chemistry

Continuous Determination of the Oxygen Dissociation Curve for Whole Blood

Luigi Rossi-Bernardi 1, Massimo Luzzana 1, Michele Samaja 1, Mario Davi 1, Daniela DaRiva-Ricci 1, Jolanda Minoli 1, Brian Seaton 1, and Robert L. Berger 1

1 Cattedra di Enzimologia, University of Milan, Via G. Celoria, 2, 20133 Milan, Italy.

We report here the development of a new method that allows continuous determination of the oxygen dissociation curve for microsamples (600 µl) of whole blood under conditions of pH, pco2, methemoglobin concentration, and 2,3-diphosphoglycerate content closely approaching those found in the circulatory system. The method consists of gradually oxygenating a blood sample by adding H2O2 in the presence of catalase (EC 1.11.1.6), to produce the reaction H2O2 rarr H2O + frac12 O2. Because the total oxygen content of blood can be derived from the known rate of H2O2 addition and the po2 is determined in the liquid phase by an oxygen electrode, the two functions (total O2 content) and (% oxygen saturation) vs. po2 are simple to calculate. pco2 and pH are controlled by adding base simultaneously with the gradual oxygenation of blood. The method described thus avoids the direct measurement of oxygen saturation of whole blood.


Key Words: catalase-produced reaction • sickle cell anemia • respiratory function of blood

Submitted on May 27, 1975
Accepted on August 13, 1975




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Copyright © 1975 by the American Association for Clinical Chemistry.