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Clinical Chemistry 21: 1810-1812, 1975;
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Clinical Chemistry, Vol 21, 1810-1812, Copyright © 1975 by the American Association for Clinical Chemistry

Sodium Iodoacetate as an Antiglycolytic Agent in Blood Samples

Edward P. Marbach 1, Monty McLean 2, Marilyn Scharn 3, and Tom Jones 4

1 Bio-Consultants, Inc., 36 S. Chester Ave., Pasadena, Calif. 91106.
2 Beckman Instruments, Inc., 2500 Harbor Blvd., Fullerton, Calif. 92634.
3 Fairmont Laboratories, 50 Bellefontaine, Pasadena, Calif. 91105.
4 St. Jude Hospital, 101 E. Valencia Mesa Dr., Fullerton, Calif. 92632.

We evaluated the effect of sodium iodoacetate on glycolysis in a series of randomly selected blood samples from patients. Glucose values for serum and for serum with added sodium fluoride (2.5 g/liter) or sodium iodoacetate (2 g and 0.5 g/liter) were compared at room temperature. Respective declines in glucose values averaged 170, 40, 30, and 30 mg/liter after 24 h. Iodoacetate-preserved (0.5 g/liter) samples showed no visible hemolysis. Results of determinations of urea with urease and of other tests on SMA 12/60 (Technicon) panels were unaffected.

Submitted on June 11, 1975







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Copyright © 1975 by the American Association for Clinical Chemistry.