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Clinical Chemistry 21: 1812-1814, 1975;
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Clinical Chemistry, Vol 21, 1812-1814, Copyright © 1975 by the American Association for Clinical Chemistry

An Example of Lyophilized Protein-Based Materials Not Simulating Patient Sera

Bernard W. Steele 1, Donald F. Koehler 1, Thomas P. Blaszkowski 2, and Miguel Azar 1

1 Clinical Laboratory Service, Veterans Administration Hospital, Minneapolis, Minn. 55417; and Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minn. 55455.
2 National Heart and Lung Institute, National Institutes of Health, Bethesda, Md. 20014.

Use of reconstituted lyophilized protein-based materials in the clinical laboratory is partly based on the assumption that these materials adequately simulate patients' sera. We examined several of these materials and found that certain ones do not have the same adsorbancies at 340 and 380 nm as do most sera. The implication of this is examined with respect to glucose determination by the hexokinase method on a dual-wavelength blank-subtraction instrument.


Key Words: ABA-100 analyzer • variation, sources of • quality control

Submitted on May 19, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.